张纯喜  副研究员

                     

(Chunxi Zhang, Associate Professor）
Tel: 010-82617053; Fax: 010-82617315；Email: chunxizhang@iccas.ac.cn

简历：1971年5月出生。1994年毕业于哈尔滨工程大学化学工程专业；1997年在中国科学院福建物质结构研究所获硕士学位，硕士期间从师于刘秋田研究员进行固氮酶的化学模拟研究；2000年在中国科学院植物研究所获博士学位，博士期间从师于匡廷云院士进行光合作用研究；2000～2004年先后在瑞典Lund大学Prof. Stenbjorn Styring小组和法国CEA Saclay原子能研究中心Prof. A. William Rutherford小组进行博士后工作。2004年至今在光生物组工作。

研究兴趣：氧化-还原酶的结构和功能及其化学模拟；光合作用及其化学模拟体系中的电子转移、质子转移和金属催化反应的微观机制；太阳能电池；人工光解水制氢；光医疗。

特征研究方法：低温电子顺磁共振（EPR）

研究内容：
1997年至今，主要致力于光合反应中心的原初光化学反应和金属催化中心的催化反应的机理及其化学模拟研究(Figure  Mn簇的结构和催化机理模型（Figure¾1)。于1999年提出光合放氧催化中心 2），模型中的钙离子（Ca2＋）结合模式被2004年光系统II的X衍射晶体结构所证实。2003年首次在5K温度下从活性光系统II中观察到伴随质子转移的次级电子给体（TyrZ）的氧化。2006年发现活性光系统II中次级电子给体不与底物水分子直接作用，这一工作对前人普遍认为次级电子给体通过与水分子直接作用而调控水氧化的观点提出质疑。2007年提出新的次级电子给体调控原初光化学反应和水催化氧化的机理（Figure 3），认为强氢键在TyrZ调控过程中起关键作用。

学术论文：
1.C. Zhang, Low-barrier   A new model for¾hydrogen bond plays key role in active photosystem II  photosynthetic water oxidation, Biochim. Biophys. Acta, Bioenergetic 2007, 1767: 493-499.
2.C. Zhang, Interaction between tyrosineZ and substrate water in active photosystem II, Biochim. Biophys. Acta, Bioenergetic 2006, 1757: 781-786.
3.C. Fufezan, C. Zhang, A. Krieger-Liszkay, A. W. Rutherford, Secondary quinone in photosystem II of Thermosynechococcus elongatus: Semiquinone-iron EPR signals and temperature dependence of electron transfer, Biochemistry 2005, 44: 12780-12789.
4.C. Zhang, A. Boussac, A. W. Rutherford, Low Temperature Electron Transfer in Photosystem II: a Tyrosine Radical and Semiquinone Charge Pair. Biochemistry 2004, 43: 13787-13795.
5.C. Zhang, S. Styring, Formation of split EPR signals in photosystem II suggests that tyrosine Z can be photooxidized at 5K in the S0 and S1 states of the oxygen-evolving complex. Biochemistry 2003, 42: 8066-8076.
6.D. Huang, X. Zhang, C. Ma, H. Chen, C. Chen, Q. Liu, C. Zhang, D. Liao, L. Li, Aggregation of tetranuclear Mn building blocks with alkali ion. Syntheses, crystal structures and chemical behaviors of monomer, dimer and polymer containing [Mn4(mu(3)-O)(2)](8+) units, J. Chem. Soc., Dalton Trans. 2007, 680-688.
7.L. Song, J. Xie, C. Zhang, C. Li, J. Zhao, Recognition of various biomolecules by the environment-sensitive spectral responses of hypocrellin B, Photochem. Photobiol. Sci. 2007, 6: 683-688.
8.Y. Ma, J. Xie, C. Zhang, J. Zhao, Three-stage refolding/unfolding of the dual-color beta-subunit in R-phycocyanin from Polysiphonia urceolata, Biochem. Biophys. Res. Commun. 2007, 352: 787-793.
9.H. Wu, D. Zhang, L. Su, K. Ohkubo, C. Zhang, S. Yin, L. Mao, Z. Shuai, S. Fukuzumi, D. Zhu, Intramolecular electron transfer within the substituted tetrathiafulvalene-quinone dyads: facilitated by metal Ion and photomodulation in the presence of spiropyran, J. Am. Chem. Soc. 2007, 129: 6839 -6846.
10.Z. Zhang, Y. Zhao, C. Zhang, S. Bian, H. Zhou, H. Wang, H. Yin, J. Huang, Characterization of a nitrogenase CrFe protein from a mutant UW3 of Azotobacter vinelandii grown on a Cr-containing medium, Chinese Science Bulletin 2006, 51: 1729-1735.
11.B. Wu, D. Yuan, B. Lou, L. Han, C. Liu, C. Zhang, M. Hong, Dynamic formation of coordination polymers versus tetragonal prisms and unexpected magnetic superexchange coupling mediated by encapsulated anions in the cobalt(II) 1,3-bis(pyrid-4-ylthio)propan-2-one series, Inorg. Chem. 2005, 44: 9175-9184.
12.Y. Zhao, S. Bian, C. Zhang, H. Zhou, H. Wang, J. Zhao, J. Huang, Characterization of a FeMo cofactor-deficient MoFe protein from a nifE-deleted strain (DJ35) of Azotobacter vinelandii, Chinese Science Bulletin 2005, 50: 2305-2310.
13.H. Zhou, C. Zhang, Y. Zhao, S. Bian, F. Ren, H. Wang, J. Huang, The composition and distribution of metal clusters in the MoFe protein from a nifZ deletion strain (DJ 194) of Azotobacter vinelandii, Chinese Science Bulletin 2005, 50, 1342-1347.
14.C. Chen, D. Huang, X. Zhang, F. Chen, H. Zhu, Q. Liu, C. Zhang, D. Liao, L. Li, L. Sun, Aggregate manganese Schiff base moieties by terephthalate or acetate: Dinuclear manganese and trinuclear mixed metal Mn-2/Na complexes, Inorg. Chem. 2003, 42: 3540-3548.
15.C. Zhang, F. Mamedov, S. Styring, His190 might directly bridge Yz and Mn-cluster: Mechanistic consequences for water oxidation deduced from DFT calculation. The Proceeding of 12th International Congress on Photosynthesis, 18-23, August 2001, S13-011.
16.X. Zhang, C. Zhang, S. Ma, H. Xu, L. Shen, L. Li, X. Zhang, T. Kuang, Q. Zhang, The theoretical studies on the machanism of the primary electron transfer in the photosynthetic reaction center, Acta Chimica Sinica 2001, 59: 456-465
17.J. Pan, C. Zhang, Y. Gong, L. Li, N. Zhao, T. Kuang, The simulation for primary electronic donor of photosystem II in vitro, Chinese Science Bulletin 2000, 45:914~920.
18.C. Zhang, J. Pan, L. Li, T. Kuang, New structure model of oxygen-evolving center and mechanism for oxygen evolution in photosynthesis, Chinese Science Bulletin 1999, 44:2209~2215.
19.C. Zhang, J. Pan, L. Li, T. Kuang, Theoretical investigation on the peripheral ligands of oxygen-evolving center in photosystem II, Science in China (Series C) 1999, 43:337~346.
20.C. Zhang, X. Zhang, L. Li, Q. Zhang, X. Zhang, T. Kuang, The structural model of P680, Chinese Sciences Bulletin 1999, 44: 862~863.
21.C. Zhang, H. Fang, L. Li, T. Kuang, Theory study on primary reaction of photosynthetic bacteria, Science in China (Series C) 1999, 42: 155~161.
22.C. Zhang, C. Chen, H. Zhu, Y. Deng, J. Chai, Q. Liu, Syntheses and structural characterizations of Fe4S4 cubane-like cluster compounds containing cycloalkylthiolate ligands, Science in China (Series B) 1998, 28: 24~30 (in Chinese).
23.C. Zhang, Discussion for mechanism of nitrogen fixation in nitrogenase and its synthesis of manual simulation, Progress in Chemistry 1997, 9: 131~139 (in Chinese).
24.C. Zhang, H. Fan, Q. Liu, Discussion on the activation models of dinitrogen by FeMo-cofactor in nitrogenase, Progress in Chemistry 1997, 9: 265~271 (in Chinese).